HPLC法测定水和废水中的醛酮类化合物

To Detect Aldehydes and Ketones Compound in Water and Wastewater by HPLC

将水样用1mol/L柠檬酸缓冲液调节至酸性(pH=3),其中醛酮类化合物与2,4二硝基苯肼(DNPH)发生衍生化反应,生成稳定的化合物———腙,经C18固相萃取小柱富集、乙腈淋洗后,用带有二极管阵列检测器的高效液相色谱仪(HPLC)经32min完成15种醛酮类化合物定性、定量分析。当测定样品为100mL时,检测限均为μg/L级,其中丙酮最高为16μg/L,丁醛最低为1.1μg/L,一般在2μg/L～6μg/L之间。

To adjust water sample to acidity (pH=3) by 1 mol/L citric acid buffer solution, aldehydes and ketones compound can react with DNPH to get stability compound, hydrazone. After enriched by C18 solid phase extraction column and washed by acetonitrile, it was detected by HPLC within 32 min to get the analysis result of 15 kinds of aldehydes and ketones compound. When the sample's volume was 100 mL, the detection limit can get μg/L level, acetone was up to 16 μg/L, butyric aldehyde was 1.1 μg/L, others were between 2 μg/L and 6 μg/L.