水稻总DNA的快速制备

FAST AND RELIABLE EXTRACTION PROCESSES OF TOTAL DNAs FROM RICE

采用两种不同的简单方法,均不接触有毒的有机试剂,且不需液氮速冻研磨,快速地制备水稻总DNA,用于SSR、STS等检测,效果稳定可靠.其中TritonX-100法提取的DNA具有很好的完整性,可用于后续其他分子生物学操作;NaOH法提取的DNA虽然降解严重,但是及时用于PCR及相关分析同样可行.两种方法均可在短时间内处理大批量的样品,操作简单,效果可靠,适于对杂种后代进行遗传连锁分析和分子标记辅助选育时的单株检测.

Using two simple protocols, total DNAs could be obtained for molecular analyses, including SSR and STS detections. Both methods did not use organic solvents or liquid nitrogen. The way of grinding fresh (or reserved under ?20℃ plant materials in Eppendorf tube and less extraction steps made it practical to treat mass samples simultaneously. The DNAs extracted with TritonX-100 were quite intact and suitable for other molecular analyses. Although the DNAs extracted with NaOH were badly degraded with no unsatisfactory results from PCR ( or corresponding ) analyses through our experiment. The methods could well apply to molecular marker detection of hybrid progeny and supplementary screening of breeding materials. Fig 2, Tab 1, Ref 8