由一株青霉菌产生的聚乙烯醇降解酶

EFFECT OF CULTURE CONDITIONS ON PRODUCTION OF POLYVINYL ALCOHOL - DEGRADING ENZYME

从纺织污水活性污泥中筛选得到一株新型聚乙烯醇(PVA)降解酶产生菌,根据形态学特征鉴定该菌属于青霉属(Penicillium sp.),实验室编号WStt02-21.这是由霉菌产生PVA降解酶的首例报道.在考察了菌株WSH02-21基本生长和产酶特性的基础上,研究了营养条件对PVA降解酶合成的影响.通过对营养条件的单因素考察和正交试验,确定了最优培养条件为PVA 40 g L-1、葡萄糖3.0 g L-1、NH4Cl 8.0 g L-1、KH2PO4 2.0 g L-1、酵母膏1.0 g L-1、:MgSO40.5 g L-1。、CaCl2 1.0 g L-1、NaCl 0.02 g L-1、FeSO4·7H2O 0.02 g L-1,初始pH 6.4.其中PVA浓度是影响Penicilliumsp.WSH02-21合成PVA降解酶的最重要因素.采用最优化条件进行验证试验,PVA降解酶酶活(4.4 U mL-1)略高于正交试验中的的最高酶活(4.3 U mL-1).

A new strain that produced polyvinyl alcohol - degrading enzyme was isolated from activated sludge of a textile factory. Morphological characteristics show that this strain belongs to Penicillium sp. As we know, it was first reported that PVA-degrading enzyme was produced by a strain of fungi. The effect of culture conditions on the production of polyvinyl alcohol - degrading enzyme was also studied. By single-factor and orthogonal experiment methods, the optimal conditions were determined as:PVA40 gL-1, glucose 3.0 g L-1, NH4Cl 8. 0 g L-1, KH2PO4 2.0 g L-1, Yeast-extract 1.0 g L-1, MgSO40. 5 g L-1, CaCl2 1. 0 g L-1, NaCl 0. 02 g L-1, FeSO4 ?7H2 O 0. 02 g L-1 and initial pH 6. 4. PVA was proven to be the most important factor that affected the production of PVA-degrading enzyme. By applying the optimal conditions, the PVA-degrading enzyme activity was 4.4 U mL-1 , which was slightly higher than the best result from orthogonal experiment (4. 3 U mL-1). Fig 7, Tab 2, Ref 11