干血点法检测HIV-1抗体在高危人群普查中的应用

Application of dry blood method to test HIV-1 antibodies in high risk population

目的 采用干血点法检测艾滋病病毒 1型 (HIV 1)抗体。方法 用干血点法对 391例吸毒者、HIV感染的高危人群进行了HIV 1抗体的检测 ,并用常规酶联免疫吸附试验 (ELISA)进行对比 ,阳性结果用免疫印迹法(WB)进行确认。结果 391例吸毒者中 ,共检出 39例阳性 ,阳性率为 9 95 % ,干血点法与ELISA法的结果完全一致 ,结果符合率为 10 0 %。 39例阳性经WB法确认为HIV感染。结论 干血点法进行HIV 1抗体检测准确可靠 ,有较高的灵敏度与特异性 ,成本低廉 ,标本易采集 ,且样本无传染性 ,方便邮寄等诸多优点 ,可以广泛应用于流行病学调查 。

Objective To test HIV-1 antibodies by using dry blood method Methods Dry blood method was used to test HIV-1 antibodies in 391 cases of drug users and ELISA assay was used for comparison And the cases with positive reaction were confirmed with western blot assay Results Of the 391 drug users 39 cases were HIV positive,with a positive rate of 9 95% The result of dry blood method was completely identical with that of ELISA,with a 100% concurrence rate The 39 HIV positive cases were confirmed as HIV carriers by western blot assay Conclusion Dry blood method is accurate and reliable in testing HIV-1 antibodies It is sensible and specific, and as a cost effective approach it is easy for sample collection and post delivery,and so can be widely used in epidemiological surveys and anonymous family HIV testing , parti cularly in rural areas where blood collection is rather difficult