云芝液体培养过程中胞外酶与胞外多糖的变化

Changes of Extracellular Enzyme Activity and Extracellular Polysaccharide During the Submerged Culture of Corilus versicolor

研究了云芝在液体培养过程中胞外酶与胞外多糖等相关指标的动态变化。结果表明,在28℃,150r/min恒温振荡培养条件下,云芝菌丝体生物量在第12d达到最大值(6.436g/L);发酵液的pH值在整个液体培养过程中变化不大;胞外蛋白质浓度于第11d达到峰值;愈创木酚氧化酶在第12d达产酶高峰,酶活性为713.2u;多酚氧化酶分别在第12d与第18d出现产酶高峰,其酶活峰值分别是660.1u和1001.0u;胞外多糖含量的最大值(23.70mg/L)也出现在第12d。

Dynamic variation of extracellular enzyme activity ,extracellular polysaccharide and other relative target during the submerged culture of Corilus versicolor were investigated. The results showed that the maxmum of hypha growth was 6.436 g/L, appeared in the 12th days .There was not significant change of pH of the fluid medium through the whole period of the cultivation. The maxmun of extracellular protein consistencey was 1.06 mg/mL, appeared in the 11th days. The peak of guaicaal oxidase enzyme production appeared in the 12th days, the maxmum enzyme activity was 713.2 u. The peak of enzyme production of polyphenol oxidase appeared twice. The first appeared in the 12th days, and its enzyme activity was 660.1 u. The second appeared in the 18th days, its enzyme activity was 1001.0 u . Extracellular polysaccharide appeared its maxmum in the 12th days, it was 23.70 mg/L.