白皮松离体胚培养与不定芽的诱导

In Vitro Formation of Adventitious Buds from Embryos of Pinus bangeana

以白皮松成熟胚为外植体进行离体培养诱导不定芽。采用L(45)16正交试验,结果表明:基本培养基和植物生长调节剂均对白皮松胚诱导不定芽起关键作用,MS+6-BA3.5mg·L-1+NAA0.4mg·L-1+IBA0.1mg.L-1对不定芽诱导率最高,达71%,增殖系数为9。激素不利于不定芽的生长,培养基中加入1%的活性炭有利于不定芽的形成和生长。

The mature embryos of Pinus bangeana were used as explants to induce adventitious buds. The result showed that the basic medium and hormone played a determinative role in producing the adventitious buds. The optimal combination of the medium was MS with 3.5 mg·L^(-1)6-BA , 0.4 mg·L^(-1)NAA and 0.1 mg·L^(-1) IBA. The inducing rate was 71%, and the average rate of propagation was 9. Hormone had no good use to the buds growing. Adding 1% activated charcoal could promote the buds producing and growing .