大鼠弥漫性轴索损伤后神经行为变化和环孢霉素A的治疗作用

Neurobehavior changes of rats after diffuse axonal injury and the treatment effect with cyclosporin A

目的 探讨大鼠弥漫性轴索损伤 (DAI)后神经行为的变化和环孢霉素 A(Cs A)的治疗作用。方法 成年 SD大鼠 2 4只 ,随机分为正常对照组、损伤对照组和环孢霉素 A治疗组 (n=8) ,以 Morris水迷宫实验和避暗实验测定伤后不同时间点 DAI大鼠学习记忆能力的变化 ,研究 Cs A的治疗作用。结果 水迷宫定位航行试验发现 ,随训练次数的增多 ,3组大鼠潜伏期 (找到平台所需时间 )总体上呈逐渐下降的趋势 ,但Cs A治疗组潜伏期较损伤对照组缩短 (F=6 0 .4 4 ,P<0 .0 1)。水迷宫空间搜索实验证实 ,Cs A治疗组大鼠平台搜寻成绩好于损伤对照组 ,与正常对照组无差别。避暗实验示正常对照组大鼠潜伏期为 (2 92 .88± 12 .6 1) s,DAI大鼠经 Cs A治疗后 ,进入暗室潜伏期则从 (15 3.2 5± 6 9.36 ) s延长至 (2 2 4 .75± 5 5 .95 ) s(F =71.5 0 ,P<0 .0 5 )。结论 DAI发生后 ,大鼠的学习记忆能力有不同程度下降 ,Cs A治疗可使损伤大鼠的学习记忆能力明显提高。

ObjectiveTo observe rat's memory and learning behavior after diffuse axonal injury(DAI) and evaluate the treatment efficacy of cyclosporin A(CsA). MethodsTwentyfour SD rats were divided into three groups: noninjured group(n=8),vehicletreated group (n=8) to whom saline was applied and CsAtreated group(n=8). By analyzing behavior changes of rats in Morris water maze and darkavoidance test,the CsA treatment efficacy was evaluated. ResultsThe Morris water maze was used to observe rats' memory and learning behavior. During a placetraining experiment,the latency to find platform in each trial descended remarkably. Vehicletreated group exhibited acquisition deficits compared with CsAtreatment group. The latency in each trial was longer in vehicletreated group (F=60.44, P<0.01 ). With continued training,the rats' performance in CsAtreatment group was better than those in control braininjured group in crossing platform test(F=4.00,P<0.01),but no difference with the normal group. In darkavoidance test,the rats in normal control group had remembered the electricity shock very fast,and seldom entered into dark chamber. The mean latency time was (292.88± 12.61)seconds . The latency time of rats in CsAtreated group was (224.75±55.95) seconds. The latency time of rats in vehicletreated group was (153.25±69.36) seconds. The time of rats in CsAtreated group was shorter than the time of vehicletreated group animal(F=71.50, P<0.05). ConclusionThe rats' memory function is impaired after DAI. The learning acquisition and memory retention impairments are ameliorated after administration of CsA.