表皮生长因子和促性腺激素对人类卵母细胞体外成熟的影响

Influence of Epidermal Growth Factor and Gonadotrophin on the in vitro Maturation of Human Oocytes

目的 :研究表皮生长因子 (EGF)和不同浓度促性腺激素 (Gn)对未成熟卵母细胞体外成熟的影响。 方法 :将EGF添加至卵母细胞培养液中 ,观察EGF Gn联合应用与单独使用Gn对卵母细胞体外成熟的影响 ;分别改变绒毛膜促性腺激素 (hCG)和卵泡刺激素 (FSH)的浓度 ,观察不同浓度的hCG、FSH对卵母细胞体外成熟的影响。结果 :添加EGF可显著提高卵母细胞的体外成熟率 (P <0 .0 5 ) ;提高hCG和FSH的浓度对卵母细胞的培养、受精及卵裂无影响。 结论 :EGF可促进卵母细胞体外成熟 ,明显提高卵母细胞体外成熟率 ;提高Gn浓度对卵母细胞的培养无进一步的促进作用。

Objective: To study the effect of epidermal growth factor(EGF) and different concentrations of gonadotrophin (Gn) on the in vitro maturation of human oocytes. Methods: EGF was added to the in vitro culture medium in order to observe the effect of Gn combined with or without EGF on the result of in vitro maturation. The concentrations of hCG and FSH were changed respectively to observe the difference between the results. Results: Adding EGF to the culture medium improved the maturation rate of oocyte significantly(P< 0.05 ). There was no difference between the results with different concentrations of hCG and FSH in the culture medium. Conclusion: EGF can improve the results of the in vitro maturation of human oocytes by increasing the maturation rate significantly. Increasing the concentration of Gn does not influence the results of in vitro maturation.