星形细胞瘤的基因表达谱和Hierarchical聚类研究

Gene Expression and Cluster Analysis of Astrocytoma by Using cDNA Microarray

目的探讨人脑星形细胞瘤发生发展中相关基因及分类特征基因的表达。方法用含13939种人基因的BioStarH140S芯片,以正常脑及18例胶质瘤组织总RNA制备的探针杂交芯片,ScanArray4000扫描信号,提取脑及不同级别星形细胞瘤的差异基因并行生物信息分析,Hierarchical聚类提取差异基因的特征。结果星形细胞瘤中筛选出438条(3.14%)差异表达基因;信息分析与细胞信号、细胞骨架和运动、癌基因及抑癌基因等多类基因密切相关;与分类相关的特征基因有MAP7、DBCCR1、PCDHA5、KCNAB1、NAP1L2等。表达谱将星形细胞瘤分成两类,与临床组织病理分类基本一致。结论芯片是基因分析和筛选肿瘤标志性基因的有效手段,可客观分析星形细胞瘤发展及预后;分类特征基因为星形细胞瘤侵袭性和预后判断提供依据,有助于临床诊治。

Objective To study the expression of astrocytoma genes and characterization classification genes by using cDNA microarray. Methods BioStarH140S microarray (including 5 592 novel genes) were used and hybridized with probes, which were prepared for total RNA. It was extracted from 2 cases of normal adult brain tissues and 18 cases of glioma tissues. Differentially expre-ssed genes between normal tissues and glioma tissues were assayed after scanning cDNA microarray with ScanArray 4 000. The gene's characterizations were studied with Hierarchical method. Results Among the 13 939 target genes, there were 438 (3.14%) differentially expressed genes between astrocytoma and normal brain tissue. By bioinformal analysis, those genes were associated with cell signal, cytoskeleton, oncogenes and suppressor genes, etc. MAP7, DBCCR1, PCDHA5, KCNAB1, NAP1L2 genes were relevant to classification by Hierarchical method. Molecular classification was in accordance with pathology and revealed internal essence in tumorigenesis. Conclusion cDNA microarray is an available technique in screening marker genes of glioma and understanding its molecular mechanism and is helpful to diagnosis and therapy of the glioma.