囊胚培养及移植在体外受精-胚胎移植中的应用

An Application of Blastocyst Culture and Transplantation in Assisted Reproductive Technique

目的 :分析囊胚培养及移植技术在体外受精 -胚胎移植 (IVF ET)中的临床效果。方法 :将行IVF ET的 2 4例患者共 2 92个卵子采用Quin’s培养基 +10 %人血清白蛋白进行序贯培养及囊胚移植 ,与传统培养 D3(第3天 )移植进行比较 ,分析受精率、卵裂率、移植胚胎数及妊娠率 ,采用 χ2 检验进行统计学分析。结果 :囊胚培养组移植胚胎数明显低于传统培养组 (P <0 0 0 1) ,而妊娠率略有提高。并于 2 0 0 2年 2月 8日诞生了贵州省首例囊胚培养及移植的试管婴儿龙凤胎。结论 :采用适合的培养液进行囊胚培养 ,可减少胚胎移植数目 ,提高IVF ET的妊娠成功率 ,降低多胎率。

Objective: To study the clinical effects of blastocyst culture technique on the outcome of in vitro fertilization-embryo transfer(IVF-ET). Methods: 292 ova from 24 patients were fertilized and sequentially cultured in Quin’s media plus 10% human serum albumin(HSA)in vitro. In 24 IVF-ET cycles, transplantation was performed at the blastocyst stage. Conventional culture that the transplantation was done on the third day after fertilization was carried out in 28 IVF-ET cycles. The fertilization rate, cleavage rate, the numbers of embryo transfered (NET) and the pregnancy rate were compared between these two groups. Results: The NET were significantly smaller in blastocyst culture group than those in conventional culture group(P<0.001). Conclusion: Blastocyst culture is more suitable for selecting good embryos to transfer, which can increase the pregnancy rate and decrease the multiple pregnancy rate.