四逆汤对兔球囊损伤后血管平滑肌细胞增殖和凋亡的影响

Influence of Sini decoction on the proliferation and apoptosis of artery smooth muscle cells after balloon injury

目的 :观察四逆汤 (SND)对兔腹主动脉球囊拉伤后血管平滑肌细胞 (VSMCs)增殖和凋亡的影响 ,探讨VSMCs增殖和凋亡在动脉损伤后再狭窄 (RS)中的作用及SND预防PCI术后RS的可行性。方法 :建立兔腹主动脉球囊拉伤模型 ,用SND进行干预 ,电镜观察中膜和内膜增殖和凋亡的VSMCs超微结构特征。用α-actin标记VSM Cs并检测其增殖细胞核抗原 (PCNA)及细胞周期素E(cyclinE)的表达 ;Tunel法检测凋亡细胞 ,计算增殖和凋亡指数。84d亚组行腹主动脉造影观察损伤段血管的狭窄程度。结果 :SND治疗组中膜内膜VSMCs增殖程度明显轻于对照组 ,凋亡指数明显高于对照组 (P <0 0 5 ) ,而且细胞凋亡持续的时间较长 ,到术后 14d才达高峰 ,以后才逐渐下降。术后 84d亚组血管造影显示治疗组损伤段腹主动脉狭窄程度明显小于对照组 (P <0 0 5 )。结论 :四逆汤能有效抑制VSMCs增殖 ,诱导其凋亡 ,减轻血管损伤后的狭窄程度。

AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs.wasont