摘要
目的 :比较 3种异种角膜基质的免疫原性。方法 :将SD大鼠 36只随机分为 4组 ,每组 9只。 1组为对照组 ,2 - 4组为实验组。分别获取新鲜猪、兔、鸡角膜基质 ,等量湿重 2 5mg异位植入大鼠背部皮下 ,术后观察伤口愈合情况 ,并于术后 7d、14d、2 8d获取大鼠外周血 ,免疫荧光标记及流式细胞仪分析比较 3种异种角膜基质对大鼠CD4 + 、CD8+ 、CD2 5 + 、CD71+ 表型的动态影响。结果 :各组植入处皮肤无红肿、无渗出及其它改变 ,伤口愈合良好 ;各组与对照组比较 :猪角膜基质组各时相表达的CD4 + 、CD8+ 、CD2 5 + 、CD71+ T细胞比例无显著差异 (P >0 0 5 ) ;兔角膜基质组术后 7d ,CD4 + T细胞升高 (P <0 0 5 ) ,鸡角膜基质组术后 7d ,CD4 + 、CD4 + CD71+ 升高 (P <0 0 1)。结论 :3种异种角膜基质对大鼠细胞免疫原性比较 :鸡 >兔 >猪 ,猪角膜基质的免疫原性最低。
AIM: To Compare immunogenicity of three kinds of heterogenic corneal stroma. METHODS: 36 SD rats were randomized into 4 groups, each group consisting of 9 rats. Group 1 was control group. Three kinds of heterogenic corneal stroma: porcine, rabbit and chicken corneal stroma were heterotopically transplanted to subcutaneous layer of 27 (group 2-4) SD rats, respectively. The expression of CD4+, CD8+, CD25+, CD71+ on peripheral T cells was identified and analyzed by dual fluorescence flow cytometry at 7, 14, 28 days after operation. RESULTS: Compared with control group, the expression of CD4+, CD8+, CD25+, CD71+ was no significant change in porcine corneal stroma group(P>0.05), the expression of CD4+ was increased in rabbit corneal stroma (P<0.05), CD4+, CD4+ CD71+ markedly higher in the chicken corneal stroma (P<0.01) at 7 days after operation. CONCLUSION: The immunogenicity of porcine stroma is the lowest in three kinds of heterogenic corneal stroma (chicken, rabbit and porcine).
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第4期613-615,共3页
Chinese Journal of Pathophysiology
基金
广东省重点科学基金资助项目 (No .A30 2 0 2 0 10 2 )
深圳市科技局基金资助项目 (No .2 0 0 2 0 4 0 30 )
