摘要
目的 :通过体外实验的方法探讨非甾体类抗炎药吲哚美辛对人喉癌Hep - 2细胞系侵袭能力的影响。方法 :Hep - 2细胞经吲哚美辛作用后 ,撤药培养 ,绘制生长曲线 ,并用软琼脂集落形成实验检测肿瘤细胞非锚着生长能力 ,琼脂糖滴法检测肿瘤细胞移动能力 ,单层细胞侵袭实验计算肿瘤侵袭指数。结果 :吲哚美辛作用后的Hep- 2细胞软琼脂集落形成数目减少 ,细胞移动能力下降 ,单层细胞侵袭指数降低。结论 :吲哚美辛能抑制人喉癌Hep- 2细胞系的侵袭性。
AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第4期632-635,共4页
Chinese Journal of Pathophysiology
基金
广东省自然基金资助项目 (2 0 0 3- 0 7)
教育部骨干教师基金资助项目 (2 0 0 0 - 6 5 )
关键词
喉肿瘤
吲哚美辛
肿瘤侵润
HEP-2细胞
Laryngeal neoplasms
Indomethacin
Neoplasm invasiveness
Hep-2 cells
