IL-8和TNF-α在创伤后牙髓组织中表达的实验研究

Immunohistochemical Expression of IL-8 and TNF-α in Indirect Injured Pulpal Tissue from Firearm Wound

目的 :研究IL - 8和TNF -α在狗颌面部火器伤间接损伤牙髓中的表达情况。方法 :采用直径 5 .80mm制式枪弹致伤狗下颌部的模型 ,利用免疫组化染色及定量分析方法及检测颌面部火器伤后不同时间、下颌不同牙齿的牙髓组织中IL - 8和TNF -α的动态变化。结果 :IL - 8和TNF -α在伤后牙髓组织中有阳性表达 ,并随时间的改变 ,呈动态变化过程。结论 :IL - 8和TNF -α可能参与火器性牙髓炎症发生、发展 ,并与牙髓损伤。

Objective: To provide theoretical basis and experimental evidence for diagnosis and therapy of dental pulp indirect injuries by gunshot wounds. Methods: The levels of IL-8 and TNF-α in different stages were studied using immunohistochemical method to evaluate its functions in inflammation process of dental pulps. Results: IL-8 and TNF-α expression were detected by immunohistochemical assay in postwound conditions. The results showed that positive staining of IL-8 and TNF-α were observed predominantly on infiltrating inflammatory cells, vasculars endothelial cells and some fibroblasts, which was consistent the recruitment and activation of inflammatory cells, and with the severity duration of pulpal tissue injury and response. Conclusions: These results indicates that IL-8 and TNF-α are involved in regulating physiologic responses of dental pulp injuries in maxillofacial trauma by a projectile.