热休克反应对大鼠中暑休克的影响及意义

Effects and significance of heat shock response on heat stroke in rats

目的 探讨热休克反应 (HSR)对大鼠中暑休克的影响及其意义。方法 SD雄性大鼠随机分为 3组 :热休克组(HSgroup) ,热休克对照组 (HSCgroup) ,正常对照组 (NCgroup)。HS组给予热休克预处理 ,而HSC组则否。两组常温恢复2 0h后予以高温热暴露处理 ,其间监测记录血压、心电 ;热暴露至 73min时终止实验 ,检测血清丙二醛 (MDA)、一氧化氮(NO)含量 ;运用Chart软件 ,获取平均动脉压 (MAP)、收缩压 (Ps)、舒张压 (Pd)、心率 (HR)等数据。NC组不予任何处理 ,以获得上述数据的正常值。结果 ( 1)热暴露 73min时 ,HS与HSC组动物的MAP、Ps、Pd值明显低于NC组 (P <0 0 1) ,HR值高于NC组 (P <0 0 1) ;HS与HSC组相比表现出更高的MAP、Ps、Pd(P <0 0 1)、HR值 (P <0 0 5 )。 ( 2 )热暴露 73min时 ,HSC组血清MDA、NO含量显著高于NC组 (P <0 0 5 ,P <0 0 1) ,而此时HS组血清MDA含量明显低于HSC组 (P <0 0 5 ) ,并与NC组差异无显著性 ,其血清NO含量亦较HSC组明显为低 (P <0 0 1)。结论 HSR能减轻大鼠中暑休克的发生 ,其机理可能与抑制MDA。

Objective To study the effects and significance of heat shock response (HSR) on heat stroke (HS).Method SD male rats were randomly divided into three groups,i.e., HS group,HS control group(HSC)and normal control group(NC).Rats in HS group were pretreated by heat and those in the HSC group were not.Twenty hours later,the rats in HS group were placed in a heat cabin for 73 minutes to cause them HS and blood pressure and electrocardiograph were monitored for them during they stayed in the heat cabin.Serum levels of malonyl dialdehyde (MDA) and nitric oxide(NO) were measured for the rats after heat exposure.No treatment for the rats in NC groups was given.Their mean arterial pressure (MAP),systolic pressure (SP),diastolic pressure(DP),heart rate (HR) were obtained for all the rats by Chart software.Result (1) MAP,SP and DP in HS and HSC groups were significantly lower than those in NC,P<0.01,and HR was higher in HS and HSC than that in NC,P<0.01.MAP,SP,DP and HR in HS group were significantly higher than those in HSC,P<0.01 and P<0.05,respectively.(2)Serum levels of MDA and NO were significantly higher in HSC than those in NC,P<0.05 and P<0.01,respectively,at 73 minutes after heat exposure,and serum levels of MDA was significantly lower in HS group than those in HSC one,P<0.05,but not significantly differing from those in NC one.Content of NO in HS group was also lower than in HSC group(P<0.01).Conclusion HSR could reduce occurrence of heat stroke in rats,possibly by inhibiting over synthesis of MDA and NO.