乙二醛对小鼠脂质过氧化物水平及SOD活性的影响

Effects of glyoxal on the serum level of lipid peroxide and activity of superoxide dismutase in mice

目的 测定乙二醛染毒小鼠血清及组织中脂质过氧化主要产物丙二醛 (MDA)的含量和超氧化物歧化酶(SOD)活力的变化 ,以探讨乙二醛毒性的可能机制。方法 40只小鼠分成 1个对照组和 3个实验组 ,实验小鼠的染毒剂量分别为 1 2 9mmol/kg、 2 5 8mmol/kg和 5 16mmol/kg ,每天腹腔注射 1次 ,连续染毒 3 0d。用硫代巴比妥酸 (TBA)比色法测定血清及组织中MDA含量 ,用黄嘌呤氧化酶法测定全血及组织中SOD活性。结果 乙二醛染毒小鼠高剂量组肾脏MDA含量与对照组相比显著升高 ,其他各项指标与对照组相比差异均无显著性 ;小鼠全血、肝脏和肾脏中SOD活性变化与对照组相比差异均无显著性。结论 乙二醛能够增高小鼠肾脏MDA含量 。

Objective To study the possible mechanism of glyoxal poisoning in mice.Method Forty mice were divided into trial and control groups.Mice in trial group were exposed to varied doses of glyoxal,1.29 mmol/kg,2.58 mmol/kg and 5.16 mmol/kg,respectively,by intraperitoneal injection once daily for 30 days.Serum and tissue levels of malonyl dialdehyde(MDA)were measured with thiobarbiturate colorimetry and activity of superoxide dismutase(SOD)in the whole blood and tissues were measured with xanthine oxidase method.Result Level of MDA in the kidney of the mice exposed to high-dose-glyoxal increased significantly than that in the control mice.There was no significant difference in the other indicators measured between the exposed and control mice.Also,there was no significant difference in activity of SOD in the whole blood,liver and kidney between the exposed and control mice.Conclusion Glyoxal could increase content of MDA in the kidney of mice exposed to glyoxal,suggesting it could cause oxidative injury in renal tissue of mice.