赤链蛇达氏腺内分泌细胞免疫细胞化学

Immunohistochemical localization of endocrine cells in Duvernoy's gland of Dinodon rufozonatum

目的 :研究赤链蛇达氏腺内分泌细胞类型、分布特点。方法 :用免疫细胞化学ABC法。结果 :发现赤链蛇达氏腺内有 8种物质均免疫反应阳性细胞 ,分别为 5 -羟色胺、生长抑素、P物质、胰高血糖素、胃泌素、表皮生长因子受体、上皮膜抗原及睾酮 ,以 5 HT数量最多 ,GAS细胞最少 ,各细胞分布部位也有所不同。结论 :赤链蛇达氏腺的各种内分泌细胞功能与蛇类取食习惯及其他蛇类特有生理活动相适应。

Objective: To study the cell types and distribution in Duvernoy's gland of Dinodon rufozonatum.Methods: 5-HT,SS,SP,GLU,GAS,EGFR,EMA,and TES positive immunoreaction were found in Duvernoy's gland,and the largest number was 5-HT cells and the smallest number was GAS cells. Moreover,the distribution of endocrine cells was different.Conclusion: Dinodon rufozonatum endocrine cells function in Duvernoy's gland is adapted to the feeding habit and special physiological action of snakes.