摘要
目的 探讨胶质瘤中血管内皮生长因子 (VEGF)受体KDR和FLT 1的基因表达水平及其与肿瘤病理的关系。方法 应用逆转录PCR(reversetranscriptionpolymerasechainreaction ,RT PCR)方法检测 4 3例胶质瘤和 5例正常脑组织标本中KDR和FLT 1的基因表达情况 ,结合凝胶分析仪半定量的检测肿瘤标本中特异性mR NA的含量 ,并与肿瘤的临床病理进行比较。结果 电泳结果 :FLT 1在 6 4 5bp左右可见一明亮的条带 ;而KDR在1 0 0 0bp以上 (1 2 4 1bp)可见一明亮的条带 ,其亮度均随肿瘤级别的升高而升高。FLT 1mRNA的相对含量Ⅰ～Ⅳ级依次为 :0 .6 4± 0 .0 8、0 .86± 0 .1 0、1 .2 3± 0 .0 6、1 .37± 0 .0 6 ;正常脑组织为 0 .34± 0 .0 4。KDRmRNA的相对含量Ⅰ～Ⅳ级依次为 :0 .70± 0 .0 8、0 .92± 0 .1 1、1 .6 2± 0 .36、2 .86± 0 .30 ,正常脑组织为 0 .4 0± 0 .0 7。VEGF两种受体mRNA的相对含量随肿瘤级别的增加而增加 ,有显著性差异 (P <0 .0 0 0 1 )。结论 两种受体之间的差异明显 ,总的来说 ,KDR要比FLT 1的mRNA含量高 ,而随着胶质瘤恶性程度的增加 ,这种差别明显增大 ,尤其在胶质母细胞瘤中 ,KDR的平均相对mRNA含量是FLT 1的
Objective To evaluate the different expression of VEGF receptors (KDR and FLT 1) mRNA in gliomas.Methods 43 glioma specimens and 5 normal brain tissues were studied using RT PCR,and gel analysis system is used to semi quantity mRNA abundance in gliomas.These results were analyzed with pathological findings of the tumors.Results The expression of FLT 1 mRNA corresponded to amplification product 645bp.The relative mRNA abundance (ratio of intensity of FLT 1/β actin) was:grade Ⅰ 0.64±0.08,grade Ⅱ 0.86±0.10,grade Ⅲ 1.23± 0.06 ,grade Ⅳ 1.37± 0.06 ,normal brain tissues 0.34±0.04.The expression of KDR mRNA corresponded to amplification product of 1241 bp.The mRNA relative concentration is grade Ⅰ 0.70±0.08,grade Ⅱ 0.92±0.11,grade Ⅲ 1.62±0.36,grade Ⅳ 2.86±0.30,normal brain tissues 0.40±0.07.Conclusions The relative mRNA abundance of the two VEGF receptors was increased with the grade of gliomas.Statistics with one way ANOV, P <0.001.It indicates that the difference of VEGF receptors mRNA expression between different grade gliomas is significant.
出处
《北京医学》
CAS
北大核心
2004年第1期10-13,共4页
Beijing Medical Journal
