氯胺酮对缺血/再灌注脑损伤小鼠海马核转录因子-κB的影响

Effect of ketamine on NF-κB in mice hippocampus following ischemic brain damage

目的 研究氯胺酮对缺血 /再灌注脑损伤小鼠海马NF -κB及大脑梗死体积的影响。方法 昆明纯种小白鼠共 30只 ,随机分为 4组 ,S组 (n =7)为假手术组 ,C组 (n =8)为缺血 /再灌注损伤对照组 ,I组 (n =8)为线栓缺血梗死后氯胺酮治疗组 ,R组 (n =7)为线栓缺血梗死再灌注后氯胺酮治疗组。结果 S组海马区NF -κB蛋白表达明显高于C组、I组和R组 ,C组、I组、R组间无明显差异。C组脑梗死体积明显高于I组和R组。C组、I组、R组的神经功能缺失体征评分无差异。结论 氯胺酮可降低缺血 /再灌注脑损伤小鼠大脑梗死体积 ,对脑损伤有保护作用 ,对海马区NF -κB蛋白表达无影响。

Objective To observe the effect of ketamine on NF-κB and volumes of infarction in rats following ischemia brain damage Methods 30 KunMing mice were randomly divided into four groups Seven mice undergwent sham operation(s group). In group C,eight mice were control group of ischemia and reperfusion In group I,the right middle cerebral artery of eight mice were occluded by nylon thread embolizing and the rats were treated with ketamine while the common carotid artery were clamped In group R,seven mice underwent ischemia and reperfusion,and then were treated with ketamine Results The NF-κB of mice hippocampus were more in group S than in group C,I and R,but there was no difference between group S,I and R The neurological scores had no difference between C,I and R groups The volumes of infarction were more in group C than group I or group R Conclusion Ketamine can reduce the volumes of infarction following ischemic brain damage, but it hasn't an effect on the expression of NF-κB in mice hippocampus after ischemia-reperfusion injury