脑缺血/再灌注后缺血核心区皮质内MCP-1 mRNA和蛋白质表达

Study of MCP-1 mRNA and protein levels in the ischemic core of rat cortex after transient focal cerebral ischemia

目的 观察大鼠脑缺血 /再灌注后缺血核心区皮质内单核细胞趋化蛋白 (MCP) - 1mRNA和蛋白质表达情况。方法 用半定量的逆转录PCR(RT -PCR)法测定缺血 2h再灌注不同时间缺血核心区脑皮质内MCP - 1mRNA的表达 ,ELISA法测定MCP - 1含量的变化。结果 缺血核心区脑皮质内MCP - 1的mRNA表达于缺血 2h明显升高 ,再灌注后 16h达高峰(均值是缺血 2h组的 2 2倍 ,与缺血 2h组相比差异显著 ,P <0 0 1) ,此后仍维持较高水平的表达直至再灌注后 4 8h(与假手术组相比 ,P <0 0 5 ) ;MCP - 1含量于再灌注后 6h开始升高 (均值 2 2 5ng/g组织 ,是假手术组的 17 0倍 ,P <0 0 5 ) ,此后逐渐升高 ,4 8h达到高峰 (均值 110 9ng/g组织 ,是假手术组的 83 7倍 ,P <0 0 1)。结论 脑缺血 /再灌注后缺血核心区皮质内MCP - 1的mRNA和蛋白质表达均明显增加 ,提示MCP - 1在脑缺血

Objective To study the mRNA and protein levels of monocyte chemoattractant protein (MCP)-1 in the rat cortical ischemic core after transient focal cerebral ischemia Methods The thirty-six male Wistar rats were subjected to the right middle cerebral artery occlusion for two hours,then killed at 0,6,16,24 and 48 hours after reperfusion The mRNA level of MCP-1 was observed by half-quantitative RT-PCR The protein level of MCP-1 was measured by enzyme linked immunosorbent assay(ELISA) Results The mRNA level of MCP-1 was increased at 2 hours after ischemia,and peaked at 16 hours till 48 hours after reperfusion The protein level of MCP-1 was markedly increased at 6 hours in the ischemic core of cerebral cortex,and peaked at 48 hours after reperfusion Conclusion The mRNA and protein levels of MCP-1 increase in rat cortical ischemic core after transient focal cerebral ischemia which suggests that MCP-1 may play an important role in the cerebral ischemia and reperfusion injury