摘要
以酪软酸钠为原料,采用混合蛋白酶水解,本文对酶水解工艺参数,小分子肽得率变化进行了系统的研究。本文得出结论:碱性蛋白酶和中性蛋白酶混合使用酶解酪朊酸钠的最适宜条件为:温度为45,pH为6.5,酶解时间为24小时,底物浓度为10%,酶用量为1.55?04u/g.
The technology of enzymatic hydrolysis was introduced. Material is sodium caseinate, and the optimum hydrolysis parameters of protease, the analysis short peptides percentage of hydrolysis protein were systematically investigated. Conclusion as follow: hydrolysis temperature 45, pH 6.5, hydrolysis time 24 hour, substrate concentration 10%, enzyme quantity 1.55?04u/g.