摘要
采用液体发酵培养的方法获得香菇发酵液(LEL),发酵液经离心,提取得胞内粗多糖(LEPI),再经DEAE柱层析得到一种蛋白多糖复合物,然后用Supdex200凝胶层析显示为单峰。
In this paper, fermentation liquor of Lentinus edodes(LEL)has been obtained by submerged fermentation, intracellular polysaccharide(LEPI)has been separated with method of centrifugation and pick-up from the Fermentation liquor, purified with DEAE-cellulose column and obtained a kind of proteoglycan, LEPI was proved to be simplex material through Supdex200 chromatography.
