摘要
克仑特罗经偶氮化后,与牛血清白蛋白在碱性条件下发生偶联反应生成偶联物。偶联物经红外光谱、紫外光谱、十二烷基磺酸钠-聚丙烯酰胺凝胶电泳、快速蛋白液相色谱及免疫鉴定,确定偶联成功。对上述几种偶联物鉴定方法进行了简单比较,认为快速蛋白液相色谱是鉴定偶联物的最佳方法。
A conjugate prepared through the direct coupling of diazotized clenbuterol and bovine serum albumin was identified by IR, UV、SDS-PAGE、FPLC and immunological experiments. A brief assessment of each analytical method was given.Among all the (analytical methods), FPLC is the best one by comparison.
出处
《中国兽药杂志》
2004年第3期22-25,共4页
Chinese Journal of Veterinary Drug
