黄芩对去污染小鼠体内痢疾杆菌R质粒消除试验研究

Studies on curing R plarmid of SH fle xneri F13 strain with Huang qin in decontaminated whitemice

本文以去污染的瑞士小白鼠为模型用黄苓对福氏痢疾杆菌F13株的R质粒进行了体内消除试验。选用红霉素、四环素和利福平对小鼠肠道进行去污染,降低其定居阻力,然后用福氏痢疾杆菌F13株经口感染,同时利用四环素造成一种选择压力,以利于对四环素耐药的F13株菌生长繁殖。利用小鼠肠道菌群重建的间隙,进行R质粒的消除试验。结果表明,黄芩对福氏痢疾杆菌F13菌株R质粒在体内具有一定的消除作用。

A method was establshed for curing R plasmid from SH klexneri F13 str ain in vivo, using the decontaminated swiss wite mice as a model. We treated micewith approprierate doses oftetr acycline, esythromycine and rifampi n. The intestinal bacteriae wereelminated,there by resulting in greatly dec rearing the coloniZation reristance of intestine of mice, thus auowing coloni zation and propagation of F13 strain in smau intertine of mice. Curing e xeperiments were carrie dout in the interval after clearing of most of norm al flara and the time before restoration of those disappeared in the traated mice _ sBch experiments showed Baicalein in vivo could cure some R plasmid in SH flexneci F13 strain residing the decontaminated mice