注射肾上腺素模拟“气滞”复制急、慢性血瘀模型初探

Preliminary Exploration on Establishing A Simulated Model of Acute and Chronic After-Qi-stagnation Blood Stasis by Adrenaline Injection

目的 验证中医情志致病“气滞血瘀”理论,探讨用肾上腺素(Adr)制造急、慢性动物血瘀模型的方法。方法 将Wistar大鼠分为5组,即空白组(不造模)、A大组(用0.1％Adr一次皮下注射)、A小组(用0.01％Adr每日1次,连续7日皮下注射)、丹参组(用0.1％Adr一次皮下注射,并用丹参治疗)和川芎组(用0.1％Adr一次皮下注射,并用川芎治疗);Adr模拟人体大怒及焦虑状态,制造大鼠血瘀模型,应用中药丹参、川芎反证证型,检测某些血液流变学指标。结果 A大组、A小组与空白组比较,低切度率下全血粘度增加(P<0.01),红细胞变形指数增高(P<0.05,P<0.01),红细胞聚集指数增高(P<0.05或P<0.01)。丹参组与A大组比较,离体血栓长度增加(P<0.01),干重、湿重增加(P<0.01),川芎组与A大组比较,差异无显著性。结论 一次大剂量及多次小剂量皮下注射Adr模拟大怒及焦虑状态可以造成动物血瘀模型,且为中医“气滞血瘀”理论提供了实验依据。

To testify the TCM theory on 'qi-stagnation causes blood stasis' and explore the method of using adrenaline to establish an acute and chronic blood stasis animal model. Methods Wistar rats were divided into 5 groups, the blank group (un-modeled), the model group A (modeled with once subcutaneous injection of 0.1% adrenaline) , the model group B (modeled with once a day of subcutaneous injection of 0.01% adrenalinei for consecutive seven days), the Salvia group (treated with salvia after modeling) and the Chuanxiong group (treated with Chuanxiong after modeling). The model was established by simulating the anger and anxious status to form acute and chronic blood stasis model respectively and the treatments of Salvia and Chuanxiong was used to testify the syndrome type. Hemorrheologic parameters of the rats were measured. Results Compared with that in the blank group, the low sheared rate of whole blood viscosity increased, and the RBC deformability and aggregation index raised in both model group A and B (P<0.01 or P< 0.05). Compared with that in Group A, in the Salvia group, the in vitro thrombus lengthened (P<0.01), with its dry weight, wet weight increased (P<0.01) after treatment, but the above-mentioned parameters in the Chuanxiong group were not changed at all. Conclusion One time large dosage and multiple times of small dosage adrenaline subcutaneous injection to simulate angry and anxious status could produce blood stasis animal model. This fact provides an experimental basis for 'Qi stagnation causes blood stasis' theory in TCM.