大肠杆菌L型生物学特性及致病性的研究

STUDIES ON THE BIOLOGICAL CHARACTERISTICS AND PATHOGENICITY OF ESCHERICHIA COLI (E.COLI) L-FORMS

我们对人工诱导的大肠杆菌L型从生物学特性、致病性以及耐药性R质粒传递等方面进行了初步的研究。结果表明:L型菌从菌体形态、菌落特征、生化反应等方面与原型菌有较大差别;原型大肠杆菌和相应的L型菌接合试验均阳性,但后者接合频率较前者明显低;将L型菌经膀胱和尾静脉感染小鼠,引起实验鼠脏器的间质性炎症,通过病理学和细菌学检查证明,L型菌可直接引起脏器感染,并非只有返祖后才能致病。

In this paper, the preliminary studies on the biological characteristics, pathogenicity and transfer of R plasmid were carried out in the artificially induced E.Coli L-forms. The results showed that the L-forms differed greatly from their parental types in the cellular morphology, colonial characteristics and biochemical activities. Conjugation tests of both parental bacteria and their L-forms were positive, but conjugation frequency in the latter was markedly lower than that in the former. After L-forms of E.Coli were injected into the urinary bladders and the tail veins of mice, the interstitial inflammations were induced in some organs of the experimental mice (lung, liver and myocardium) . Using-bacteriological and pathological methods, we verified that L-forms of E. Coli themselves could cause infections without prerequisite of their reverting back to the parental bacteria.