摘要
造血细胞磷酸酶 (HCP)在造血细胞发育、增殖及受体介导的有丝分裂信号传导通路中发挥关键的负调节作用 ,在motheaten小鼠中其突变可导致粒 单核细胞严重的过度聚积和功能紊乱。本研究旨在评价HCP基因突变在急性白血病发病中的作用。利用RT PCR ,SSCP及DNA序列分析技术检测了 4 1例急性白血病、8株白血病细胞系及 5 0例正常对照骨髓或外周血标本中HCP基因表达及突变情况。RT PCR显示所有标本中都有HCP基因表达 ,仅在 1例急性淋巴细胞性白血病细胞中发现一错义突变 ,发生在HCP基因氨基末端的SH2结构域 ;此外 ,分别在HCP基因的 6 9,85 ,86和 2 6 6密码子存在多态性。结论 :HCP基因突变在急性白血病中较少见 ,在白血病发病中可能起较小作用 ,需进一步研究澄清。
The hematopoietic cell phosphatase (HCP or SHP 1), the SH2 domain contain protein tyrosine phosphatase, is a crucial negative regulator in the process of hematopoietic cell development, proliferation and receptor mediated mitogenic signaling pathways, and its mutation is responsible for the over expansion and inappropriate activation of myelomonocytic population in motheaten mice. The aim of the study was to evaluate the role of the HCP gene in leukemogenesis. Bone marrow and/or peripheral blood from 32 acute myeloid leukemia (AML) patients, 9 acute lymphocytic leukemia (ALL) patients, 8 leukemia cell lines and 50 normal controls were analyzed by reverse transcription polymerase chain reaction (RT PCR) based on single strand conformation polymorphism (SSCP) and sequencing. RT PCR showed that all samples expressed HCP gene, only one missense mutation at codon 225 (AAC to AGC, Asn to Ser) within N terminal SH2 domain was found in an ALL patient. In addition, four polymorphic base substitutions were detected in codon 69, 85, 86 and 266, respectively. In conclusion, mutation of HCP gene is an infrequent genetic aberration which may only play a role in pathogenesis of a small part of leukemia, however, its significance needs to be further clarified.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第2期128-132,共5页
Journal of Experimental Hematology