mPEG修饰解决血型匹配困难的体外研究

Preliminary in vitro Study on Modification of RBC with mPEG-BTC for Resolving the Problem of Clinical Difficulty in Blood Matching

本研究的目的是通过mPEG修饰红细胞表面抗原以期找到一种解决临床疑难配血的方法。收集了含高效价抗体及发生临床配血困难病例的血清共 2 9例 ,分析了病因及所含的抗体 ,并采用 1.0mmol/L的mPEG BTC与献血者红细胞混合 ,2 5℃孵育 1小时 ,用聚凝胺法和抗人球蛋白法检测对比mPEG修饰前后的红细胞与 2 9例血清的凝集情况。结果表明 :临床配血困难及血清含高效价抗体的病例主要发生在血液系统疾病和肿瘤及新生儿溶血病产妇 ,所含的抗体主要是Rh血型系统的抗体和自身抗体。 1.0mmol/LmPEG BTC修饰的红细胞后 ,不会与这些病例血清中的抗体发生反应 ,达到了临床配血输用的要求。结论

The aim of this study was to find an effective solution for difficulty of blood matching. Twenty nine cases with clinical difficult in blood matching were collected, classified by their etiological factors, and analyzed with all the antibodies in serum. RBC from health donor were incubated with mPEG BTC at 25℃ for 1 hour. The coagulation of patient serum and donor RBC before and after mPEG BTC camouflage was detected and compared by polybrene and antihuman globulin reagents. The result showed that 29 cases with difficult blood matching mainly suffered form blood diseases and tumors. The main antibody were Rh and autoantibody. Donor RBC modified by mPEG showed no coagulation with the blood serum in the patients with problems of blood matching. In conclusion, the modification of RBC with mPEG BTC provides a useful strategy for resolving problem of clinical difficulty in blood matching.