二茂铁掺杂SiO_2纳米粒子修饰的次黄嘌呤生物传感器在脑渗析液分析中的应用

Amperometric sensor based on ferrocene-doped silica nanoparticles as electron transfer mediator for the determination of hypoxanthine in rat brain coupled to in vivo microdialysis

该文以二茂铁为核,二氧化硅为壳,利用反相微乳液技术,通过正硅酸四乙酯的水解制备了掺杂有二茂铁的二氧化硅(FcDS)纳米颗粒。将FcDS纳米颗粒与黄嘌呤氧化酶(XOD)混合,再通过戊二醛交联,得到以二茂铁为媒介体的次黄嘌呤生物传感器。该传感器不仅克服了传统介体型传感器中介体易流失的缺点,更提高了检测的灵敏度。实验证明,该传感器对次黄嘌呤的线性检测范围为1.0×10^(-6)～8.0×10^(-4)mol/L,检出限为5.0×10^(-7)mol/L。与微渗析技术联用,成功地用于大鼠脑中次黄嘌呤的检测,为生理以及临床医学的研究中提供了新的检测手段。

In this paper, microdialysis sampling was combined with enzymatic assay of hypoxanthine level in ratbrain. A novel amperometric hypoxanthine biosensor based on ferrocene-doped silica (FcDS) nanoparticles. Theseuniform FcDS nanoparticles (about 15 nm±3 nm) were prepared by a water-in-oil (W/O) microemulsion method,which were characterized by TEM and electrochemical technology. The nano silica surface exhibited high hiocom-pahility and the doped inside ferrocne maintained its high electron-transfer efficiency as a mediator. The hypoxanthine biosensor showed a detection limit of 5.0×10^(-7) mol/L with the linear range from 1.0×10^(-6) to 8.0×10^(-4)mol/L. Coupled to microdialysis, it was used to determine the hypoxanthine concentration in rat brain. The result was in satisfactory agreement with the references.