雄激素受体基因微卫星CAG多态与食管癌的分级

CAG Microsatellite Polymorphisms of the Human Androgen-receptor Gene and the Grade of Esophageal Cancer

目的：探讨食管癌组织中雄性激素受体(AR)基因外显子1微卫星位点(CAG)n的多态性与食管癌分级的关系,及其与肿瘤生物学特性的相关性。方法:应用PCR扩增方法,对41例食管癌组织和30例癌旁组织AR的第一外显子CAG微卫星数量进行测定,并进行不同级别间的比较分析。结果:食管癌组织AR基因(CAG)n重复范围8～28,癌旁组织n范围在8～24;食管癌组织n范围在≤10或≥22的占34.1%,比癌旁组织高(13.3%),经χ2检验有显著性差异;在29对配对的标本中,多态性发生改变的占48.3%;AR基因CAG微卫星的数量随着食管癌细胞分化程度的降低而减少,其低分化食管癌(13.6)均数低于高分化食管癌(17.5),短组AR基因CAG微卫星分布比例随着食管癌的分化程度的升高而降低,长组AR基因CAG微卫星分布比例随着食管癌的分化程度的升高而增加。结论:在食管癌组织AR基因(CAG)n重复多态的改变是高发事件,AR基因的(CAG)n微卫星数量的减少与食管癌的恶性程度相关。

Objective: to investigate the polymorphism of (CAG)n repeats within androgen receptor gene among esophageal cancer, and its possible association with esophageal cancer. Methods: The number CAG repeats in exon 1 of the AR gene were measured in 41 esophageal cancer Tissues (squamous cell carcinomas) and 30 pericancerous non-tumor tissues by PCR-PAGE analysis, and its association with grades of esophageal cancer determined. Results: The number of polymorphic (CAG)n repeats ranges from 8～28 in esophageal cancer and ranges from 8～24 in pericancerous non-tumor tissues. The number of (CAG)n repeats (≤10或≥22) differed significantly between esophageal cancer tissues (34.1%) and pericancerous non-tumor tissues (13.3%). The change of polymorphism of (CAG)n repeats in esophageal cancer Tissues is high event. The frequency of short CAG repeats dropped with the increase in tumor cell differentiation while the opposite was true for that of long CAG repeats. Conclusion: The change of polymorphism of (CAG)n repeats in esophageal cancer Tissues is high event Short CAG repeats in AR gene may signify aggressiveness.