非小细胞肺癌微切割组织中3p杂合性丢失的研究

Loss of Heterozygosity at Chromosome 3p in Microdissected Non-Small Cell Lung Cancer

目的:探讨微切割非小细胞肺癌(non-smallcelllungcancer,NSCLC)组织中3p14.2和3p25等位基因的杂合性丢失(lossofheterozygosity,LOH)与NSCLC发生发展之间的相关性。方法:采用微切割技术和聚合酶链反应(PCR)结合二核苷酸(CA)n重复序列多态性方法,分析44例NSCLC石蜡切片中癌组织和非癌组织中3p14.2和3p25位点的杂合性丢失(LOH)。结果:在44例NSCLC中29例组织标本检测3p14.2位点的LOH,其丢失率为65.5%(19/29),而另外15例患者中3p25的LOH的丢失率为40.0%(6/15)。微切割的非癌组织均未见有3p的丢失,癌组织的3pLOH频率与肺癌组织学分类及TNM分期均无明显关系(P>0.05)。结论:NSCLC癌组织中普遍出现3p14.2及3p25杂合性丢失现象,从而提示该两处可能存在一个或多个与人体肺细胞癌变相关的抑癌基因。

Objective: To investigate the frequency of loss of heterozygosity (LOH) at chromosome 3p14.2, and 3p25 in non-small cell lung cancer (NSCLC) tissues. Methods: 44 NSCLC samples were isolated using microdissection technique and DNAs were purified from tumor and non-tumor tissues section respectively. DNA samples were amplified with microsatellite markers on the following loci: 3p14.2(3S1228) and 3p25(3S1038) by using PCR. Results: It was found that 3p14.2 LOH occurred in 19 of 29 (65.5%) NSCLC samples and 3p25 LOH happened in 6 of 15(40%) respectively, while there was no 3p LOH to be observed in normal tissues. The frequency of LOH at 3p is not relative to TNM staging and pathological classification of NSCLC. Conclusion: Since 3p14.2 and 3p25 LOH occur commonly in human NSCLC, it was suggested that the two chromosomal regions might harbor one or more suppressor genes involving in the carcinogenesis of NSCLC.