人肝细胞癌VEGF及受体的表达与临床病理的关系

Relationship between Expression of VEGF、flk-1、flt-1 Protein and Clinicopathology in Hepatocellular Carcinoma

目的:分析VEGF及其受体flt-1/flk-1蛋白表达与肝细胞癌临床病理的关系。方法:应用ABC免疫组化染色法,检测60例肝细胞癌中VEGF、flk-1、flt-1蛋白表达情况,并分析它们与肝细胞癌临床病理的关系。结果:60例肝细胞癌组织中VEGF、flk-1、flt-1蛋白的阳性表达率分别为81.3%、88.3%、80.0%,均明显高于正常肝组织(P<0.05);并且VEGF蛋白表达与肿瘤的病理分级、有无包膜、瘤栓、直径大小均有相关性。结论:VEGF蛋白表达水平越高,细胞恶性程度及侵袭、转移能力越强,并且VEGF与flt-1/flk-1在肿瘤形成过程中具有协同作用。

Objectives: To study the relationship between expression of VEGF、flk-1、flt-1 protein and clinicopathology in hepatocellular carcinoma. Method: The expression of VEGF、flk-1、flt-1 protein in 60 patients with hepatocellular carcinoma were detected by immunohistochemistry (ABC method) and the relationship between expression of VEGF protein and its clinicopathology was evaluated. Results: The positive rates of VEGF、flk-1、flt-1 protein were 81.3%、88.3%、80.0% in tumor tissues, respectively, and they were significantly higher than that in normal liver tissue (P<0.05). The expression of VEGF protein was correlated with the histologic grade, metastasis of tumor. Conclusion: The results showed that the higher expression of VEGF protein was associated with the higher malignant degree and more chances of metastasis in hepatocellular carcinoma. VEGF、flk-1、flt-1 play an important role in tumourgenesis.