端粒酶与凋亡相关蛋白在卵巢交界性肿瘤中的表达

Significance of Expression of hTERT and Apoptosis Related Protein (bcl-2 and caspase-3) in Borderline Epithelial Ovarian Tumors

目的:探讨端粒酶蛋白亚单位(hTERT)与凋亡相关蛋白(bcl-2、caspase-3)在卵巢上皮性交界性肿瘤中的表达及临床意义。方法:选取天津市肿瘤医院病理科存档的卵巢上皮性交界性肿瘤41例,采用免疫组化SP法检测hTERT、bcl-2与caspase-3在肿瘤组织中的表达,与22例良性、30例恶性肿瘤进行比较,并探讨三者间表达的相关性。结果:1)hTERT在卵巢交界性肿瘤中阳性率为46.3%,而良性与恶性肿瘤分别为36.4%,73.3%,与交界性肿瘤相比,恶性肿瘤阳性率明显升高(P=0.023),而良性肿瘤与之的差异无统计学意义(P=0.446)。2)bcl-2和caspase-3在卵巢交界性肿瘤中阳性率为58.5%和73.2%,良性与恶性肿瘤分别为59.1%、72.7%和86.7%、43.3%,其中bcl-2的阳性率在交界性肿瘤中明显低于恶性肿瘤(P=0.010),caspase-3反之(P=0.011);但它们在交界性肿瘤中的表达无相关性(r=-0.063,P>0.05),且二者在交界性与良性肿瘤间的差异无统计学意义(P=0.966,0.970)。3)对93例卵巢肿瘤研究发现,hTERT与bcl-2蛋白表达无相关性(r=0.037,P>0.05);hTERT与caspase-3表达呈显著负相关(χ2=4.8109,r=-0.227,P<0.05)。结论:hTERT蛋白表达可以反映端粒酶活性与hTERTmRNA水平,与凋亡相关蛋白(bcl-2、caspase-3)的联合检测可进一步判断卵巢交界性肿瘤良恶性。

Objective: To study the significance of expression of the catalytic subunit of human telomerase hTERT and apoptosis related protein (bcl-2, caspase-3) in borderline epithelial ovarian tumors. Methods: Forty-one cases of borderline epithelial ovarian tumors were studied which were admitted to the Tianjin Cancer Institute and Hospital. hTERT, bcl-2 and caspase-3 were analyzed using immunohistochemistry in formalin-fixed paraffin-embedded tissue. Their status were compared to twenty-two benign and thirty malignant tumors. Results: ⑴ Of the borderline ovarian tumors, the positive expression rate of hTERT was 46.3% which was significantly lower than those of malignant(73.3%) tumors (P=0.023), but the difference between the borderline and benign ovarian tumors was not significant(P=0.446). ⑵ Of the borderline ovarian tumors, the positive expression rate of bcl-2 and caspase-3 was 58.5%and 73.2%, while in the benign and malignant tumors was 59.1%and 72.7%, 86.7% and 43.3%, respectively. Compared to borderline tumors ,the expression rate of bcl-2 and caspase-3 in malignant tumors was significantly higher or lower(P=0.010,0.011), but there was no correlation between expressions of bcl-2 and caspase-3 in borderline ovarian tumors(r=-0.063, P>0.05). And the differences of them between benign and borderline tumors were not significant(P=0.966, 0.970). ⑶ Of the ninety-three ovarian tumors, there was no correlation between expressions of hTERT and bcl-2(r=0.037, P>0.05); but there was significantly reverse correlation between expressions of hTERT and caspase-3 (r=-0.227, P<0.05). Conclusion: These data suggest that detecting hTERT may reflect telomerase activity and the level of hTERTmRNA, co-examination of hTERT and apoptosis related protein (bcl-2 and caspase-3) may contribute to estimating the malignant potential of borderline ovarian tumors. So they possibly become new markers to distinguish a subpopulation with the potential to develop invasive carcinomas.