杂色曲霉素对人外周血单个核细胞TAP1及LMP2基因表达的影响

Effects of Sterigmatocystin on TAP1 and LMP2 Expression of Human Peripheral Blood Mononuclear Cells in vitro

目的:探讨杂色曲霉素(ST)对体外培养的人外周血单个核细胞(HPBMc)抗原加工递呈相关基因TAP1、LMP2表达的影响。方法:采用RT-PCR和FCM方法,分析5种浓度ST处理对体外培养的HPBMcTAP1、LMP2基因在mRNA和蛋白水平表达的影响。结果:RT-PCR检测结果表明,ST处理24h后,低浓度ST组(0.125mg/L、0.25mg/L)HPBMcTAP1mRNA相对表达量明显高于对照组,而较高浓度组(0.5mg/L、1mg/L和2mg/L)TAP1mRNA则明显低于对照组,尤以1mg/L和2mg/L组为著。FCM定量分析表明,低浓度ST组HPBMcTAP1蛋白平均表达量略低于对照组,但无统计学意义,而较高浓度组TAP1表达则明显低于对照组(P<0.05)。在0.125mg/L到1mg/L浓度范围内,各ST处理组HPBMcLMP2蛋白表达量和mRNA的相对表达量均高于对照组。结论:ST对体外培养的HPBMcTAP1mRNA表达和蛋白表达的影响按ST剂量不同而变化,在0.125～1mg/L浓度范围内ST在mRNA及蛋白水平均可剂量依赖地提高体外培养的HPBMcLMP2的表达。

Objective:To explore the effects of Sterigmatocyst in(ST)on TAP1and LMP2 expression of human peripheral blood mononuclear cells ( HPBMc ) in vitro.Methods:The expressions of TAP1and LMP2of HPBMc in vitro after ST t reatment at five different concentration ( 0.125mg/L,0.25mg/L,0.5mg/L,1mg/L,2mg/L ) were studied with RT)PCR method and flow cytometry(FCM)re spectively.Results:The effects of ST on TAP1expressions of HPBMc24hrs after ST treatment showed that mRNA expressions in relatively lower ST concentration(0 .125mg/L,0.25mg/L)groups were higher than that of control group,while at relativ ely higher concentration ( 0.5mg/L,1mg/L and2mg/L ) groups,especially those in ST1mg/L and2mg/L groups,were lower than that of c ontrol.FCM quantitative anal ysis showed that at protein level,TAP1expression(as expressed by FI)of HPBMc at relatively low concentration were lower than that of control,but no statisti cal significance was found,while those of relatively higher ST concentration gro ups were all significantly lower than that of control(P<0.05).At the concentrati on range from0.125mg/L to1mg/L,the expressions of LMP2of HPBMc in ST treatment g roups at both mRNA and protein level were all significantly higher than that of the control.Conclu sion:The effect of ST on TAP1mRNA and protein expression of HPBMc in vitro was different as ST concentration changed,while ST could increase LMP2expressio n of HPBMc at both mRNA and protein level dose)dependently.