摘要
以小麦T型细胞质雄性不育系为材料,利用双向电泳技术,对苗期、分蘖期、拔节期和孕穗期叶片和花粉母细胞减数分裂期、单核小孢子期、二—三核小孢子期蛋白质变化作了分析。在细胞质雄性不育系小麦拔节期、孕穗期叶片中,有一个33KD/PI6.3蛋白组分存在,保持系中没有发现这个蛋白组分。在花粉败育的关键时期二—三核小孢子期,小麦细胞质雄性不育系有53KD/PI5.5、50KD/PI5.7、48KD/PI5.6和20KD/PI7.5四种蛋白组分存在,而保持系中也没有存在。小麦细胞质雄性不育系叶片和小孢子发育过程中存在的这五种特异蛋白可能参与育性调控,与细胞质雄性不育特性的形成有关。
By using 2D-PAGE techniques, protein compositions have been studied at different developmental stage leaf (seedling, tillering, shooting and booting stages) and pollen mother cells (PMC)(meiotic, 1-nucleus and 2-3 nuclei stages)in male sterile wheat and its maintainer. The results indicated that a specific protein (33KD/pI6.3) identified at the shooting and booting stage leaves in male-sterile wheat, but this protein species has not been identified in the male fertile wheat. Four protein species, 53KD/PI5.5, 50KD/PI5.7, 48KD/PI5.6, 20KD/PI7.5 were detected at the 2-3 nuclei stages,which was thought key stage of pollen fertility lost in male-sterile wheat, and these protein species were not be seen in male-fertile wheat. According above experiment results, five protein species which detected in leaf and PMC might participate in fertility regulation and related to the male-sterility of male-sterile wheat.
出处
《实验生物学报》
CSCD
北大核心
2004年第1期45-49,共5页
Acta Biologiae Experimentalis Sinica
基金
教育部科学技术研究重点项目(02010)
关键词
小麦
T型细胞质雄性不育系
拔节期
特异蛋白
双向电泳
育性调控
Cytopasmic male-sterile line. Shooting stage. 2-3 nuclei stage of pollen. 2D-PAGE. Specific protein