CCl_4致小鼠肝损伤中几种免疫介质含量变化的研究

CHANGES OF SOME IMMUNE-MEDIATORS IN CC1_4-INDUCED LIVER INJURY MICE

本文通过研究CCl_4致小鼠肝损伤组织匀浆和血浆一些免疫介质含量的变化以探讨这些免疫介质在CCl_4诱发肝损伤过程中作用机制。分别选用30只健康成年小鼠,雌雄各半,随机分成对照组和CCl_4负荷组,每组15只。通过腹腔注射CCl_4诱发肝损伤后,分别在第2、4、6周检测肝组织匀浆cAMP、cGMP和MDA及血浆IL-2、TNF-α水平的变化。结果显示,在整个实验期内,CCl_4组肝组织匀浆cAMP水平均低于或明显低于对照组;cGMP在实验第2周后,高于或显著高于对照组;cAMP/cGMP比值呈现下降趋势,并低于或明显低于对照组;MDA含量明显高于对照组。在整个实验期内,CCl_4组血浆IL-2水平下降或显著下降;TNF-α水平则均高于或显著高于对照组。结果提示,CCl_4负荷诱发免疫介质cAMP、cGMP、TNF-α和IL-2发生剧烈变化,在介导肝损伤过程中可能起重要作用。

Changes of some immune-mediators in liver tissue homogenate and plasma of liver injury mice injected with CC14 and its mechanism in the liver injury process induced by CC14 were studied. Thirty healthy mice with half of female and male were chosen and divided randomly into two groups which were control group (group C) and CCl4-injecting group (group CCl4).The liver injury was induced by abdominal injection of CC14 (0.07ml/100g body weight) on every other day over six weeks. The blood and liver were collected at the 2nd, 4th and 6th week, respectively, and liver homogenate cAMP, cGMP levels and MDA concentration as well as plasma IL-2 and TNF-α levels were determined. The results show that during the entire experimental period, cAMP level decreased or markedly decreased in group CC14. After the 2nd week of CCl4-in-jecting, cGMP was lower or significantly lower in group CC14 than in group C; cAMP/cGMP ratio tended to drop, and was low or significantly low to group C; and MDA content was significantly higher in group CC14 than in group C. During the whole experimental period, a marked decrease of plasma IL-2 in group CC14 was seen, and plasma TNF-α of group CC14 was superior to that of group C. It is suggested that the violent changes of immune-mediators including cAMP, cGMP,TNF-α and IL-2 etc induced by CC14 may play an important role in the induction of liver injury in mice.