纳米级全氟碳化合物抗出血性休克和神经保护作用的实验研究

Experimental study on anti-hemorrhagic shock and neuroprotection effects of nanometer perfluorocarbon

目的 研究纳米级全氟碳化合物抗出血性休克作用的有效性和安全性 ,及对脑皮质神经元的保护作用。方法 建立出血致低血容量性休克新西兰兔模型 ,观察纳米级全氟碳治疗前、后及各组各时间点心率、呼吸、血压、血气、肝肾功能等各参数及脑皮质的病理变化。结果 实验组 12h存活率比对照组高 ,呼吸较对照组深大 ,次数加快 ,监测结束时血压较对照组为高 ,动脉血氧分压较对照组明显升高 ,CO2 排泄较对照组增强 ,瞳孔对光反射较对照组灵敏 ,肝肾功能明显好于对照组 ,脑皮质损伤较对照组明显减轻 ,对照组血气呈现明显变酸趋势。结论 纳米级全氟碳化合物具有明确的抗出血性休克作用 ,无肝肾毒性 ,是一种安全有效的神经保护剂。

Objective To study the efficacy and safety of nanometer perfluorocarbon (PFC) in the treatment of hemorrhagic shock, and its role in protecting cerebral cortex nerve cells.Methods Hemorrhagic shock was induced in New Zealand rabbits by arterial bleeding. In the course of the experiment, heart rate, respiratory rate, blood pressure, blood gases, hepatic and renal function, and pathological changes in cerebral cortex were observed. Results The survival rate of animals in the experimental group at 12 hours was higher than that of control group, respiration was deeper and respiratory rate was faster, blood pressure and PaO 2 were significantly higher than that in animals of the control group. There was a tendency of acidosis in the control group. Pupil reflex was more sensitive in the experimental group, and hepatic and renal functions were better in the experimental group than that in control group. Pathological observation showed the cerebral cortex was less damaged in the treatment group than that in control group. Conclusions Nanometer PFC was effective in combating hemorrhagic shock, showing no toxicity to liver and kidney, and it could also be used as an effective neuroprotective agent.