新生大鼠嗅球纤维层组织移植促进受损视网膜节细胞存活和再生的研究

TRANSPLANTING NERVE FIBER LAYER OF THE NEONATAL RAT OLFACTORY BULB TISSUE PROMOTES THE SURVIVAL AND REGENERATION OF THE INJURED RETINAL GANGLION CELLS

许多研究表明 ,改善局部微环境可促进受损中枢神经的再生。本研究对 SD大鼠进行眶内视神经切断术作为中枢神经损伤模型 ,将新生鼠嗅球纤维层组织植入视神经断端 ,动物术后分别存活 1、2、3、4周取眼球作水平冰冻切片 ,用 Nissl染色和生长相关蛋白 (GAP-4 3 )免疫组化方法观察视网膜节细胞的存活数量和蛋白的变化 ;并观察移植物中嗅成鞘细胞的存活状况。结果显示 :植入嗅组织后可见视网膜节细胞的存活数量和存活率明显增加 (P<0 .0 5 ) ;视网膜节细胞层 GAP-4 3持续表达 ;嗅成鞘细胞可以在视神经断端存活。本研究结果提示 ,移植嗅球纤维层有促进视网膜节细胞的存活和再生的作用。

It has been suggest that providing a permissive environment can promote CNS regeneration. we used intraorbital transection of the SD rat optic nerves as a model system to investigate the effect that nerve fiber layer of the olfactory bulb exerted on the survival and regeneration of retinal ganglion cells(RGCs). The nerve fiber layer of the olfactory bulb tissue taken from the neonatal SD rat was transplanted into the stump of the optic nerve after transection, then the rat was allowed to survive for 1, 2, 3 or 4 weeks. The promoting effect was estimated by measuring the survival ratio of RGCs and GAP 43 immunohistochemical reactivity in the retina section; and by detecting whether the olfactory ensheathing cells(OECs) could survive in the bridge area. The results showed that olfactory bulb tissue transplanted in the optic stump significantly promoted RGCs survival (P<0.05) and long term up regulation of GAP 43, and that the OECs could survive in the bridge area of the optic never, suggesting that transplantation of the olfactory bulb tissue can promote the regeneration of the injured retinal ganglion cells.