短帚霉对小鼠致病性实验研究

Experimental Study on Pathogenicity of Scopulariopsis brevicaulis

目的研究短帚霉的致病性,探讨系统性真菌病的发病机制。方法给不同免疫状态的小鼠注射短帚霉菌悬液,对处死及自然死亡小鼠各脏器进行组织病理检查和逆培养。结果在接种后早期即发生各脏器侵袭,免疫受损组小鼠坏死组织出现早,脾内多形核白细胞减少。结论短帚霉是一种毒性较强的条件致病菌,真菌穿透宿主细胞的能力在感染的进程中起关键作用。多形核巨细胞在感染早期免疫应答中起重要作用。

Objective To investigate the pathogenicity of Scopulariopsis brevicaulis and to discuss the pathogenesis of systemic mycosis.Methods Cell suspension of S.brevicaulis was injected into tail-veins of normal and immunocompromised mice.Histopatholoy and fungal culture were performed on sacrified and naturally died mice at different time.Results The organs were infected soon after injection.Necrotic tissue appeared earlier and polymorphonuclear leucocytes(PMNs)decreased in the spleens of the immunocompro-mised mice.Conclusions Scopulariopsis brevicaulis is a kind of opportunistic fungus with relatively high virulence.The ability of fungi to penetrate host cells is crucial for the progression of infection.PMNs play an important role in the early immune response.