VCAM-1表达与乳腺癌血管生成及临床病理的关系

Investigation of the correlation between breast carcinoma and VCAM-1 expression in tumor or blood serum

目的 研究乳腺癌瘤体中血管细胞粘附分子 (VCAM 1)及外周血清中可溶性血管细胞粘附分子 (sVCAM 1)的表达对乳腺癌血管生长及转移的影响。方法 对 5 2例乳癌瘤体、瘤旁 (2cm)及正常乳腺组织标本 ,2 0例良性乳房纤维瘤标本进行了VCAM 1、CD34免疫组化染色 ,图像分析 ,了解VCAM 1表达、CD34的表达情况 ,并分析VCAM 1的表达与微血管密度 (MVD)的相关性 ,同时用酶联免疫吸附试验对 2 5例正常人 ,30例乳腺癌术前、术后一周外周血进行检测 ,分析瘤体及外周血清中VCAM 1的表达与乳腺癌病理参数的关系。结果 VCAM 1不仅在乳腺癌血管内皮细胞上高表达 ,在乳腺癌细胞膜上也有表达 ,而且VCAM 1的表达强度、表达率与乳腺癌的血管的生成和转移相关。乳腺癌组病人外周血清中sVCAM 1的浓度比正常对照组高 (P <0 0 0 1) ,并且术后乳腺癌病人血清中sVCAM 1的浓度下降 (P <0 0 1)。结论 VCAM 1在乳腺癌的血管生成、癌细胞转移中可能起到重要作用 ,sVCAM 1有望成为乳腺癌的早期诊断及判断复发的指标。

Objective To study the correlation of expression of VCAM-1(Vascular cell adhesion molecule-1)in tumor of breast cancer and circulating sVCAM-1(soluble vascular cell adhesion molecule-1)with angiogensis,tumor metastasis,clinical,histological and pathological parameters.Methods Specimens from 52 breast cancer patients(cancer,adjacent-cancer and normal tissue),20 breast fibroma were stained.Image analysis with the methods of immunohistochemistry followed.The relation of the data collected with clinic and pathology of breast cancer was studied.The correlation between VCAM-1 expression and pathological characteristics of breast cancer was studied.Meanwhile,the serum levels of soluble VCAM-1 were measured with enzyme linked immunosorbent assay in 25 normal people and 30 patients with breast cancer preoperatively and postoperatively.The correlation between sVCAM-1 expression and pathological characteristics of breast cancer was studied. Results VCAM-1 was expressed not only in vascular endothelial cells,but also in the membrane of some breast cancer cells.Serum concentration of VCAM-1 was elevated in the patients with breast cancer in comparison with a group of healthy subjects( P< 0.001),and the level of postoperative sVCAM-1 was reduced significantly on contrast to preoperative( P< 0.01).Conclusions VCAM-1 may play a crucial role in the angiogenesis and metastasis,and sVCAM-1 may be considered as an effective diagnostic and prognostic factor.