彰武松起源的研究

On Origin of Pinus Densiflora Var Zhangwuensis

用随机扩增多态性DNA(RAPD)技术对章古台地区的樟子松、油松、赤松及彰武松进行了基因组多态性分析,共选用24个10 bp随机引物扩增出147个DNA片段,其中,樟子松和彰武松显示出自身特征性标记,利用这些片段进行种间遗传关系分析,根据UPGMA方法构建聚类树状图研究结果表明:赤松×油松的天然杂交是彰武松形成的主要机制.

The genomic DNA variations of P.sylvestris var. mongolica, P.tabulaeformis, P.densiflora, P.densiflora var. zhangwuensis of Zhanggutai area were analyzed by using random amplified polymorphism DNA(RAPD) in the present study. Twenty-four decanucleotide arbitrary primers generated 147 RAPD bands, these fragments were used in the analysis of the relationship of 4 species. The dendrogram was constructed by using UPGMA method based on their genetic distance. The dendrogram based on the genetic distance supports the hypothesis that P.densiflora var. zhangwuensis originated from the nature hybrid of P.densiflora and P.tabulaeformis.