沙尘暴PM_(2.5)、PM_(10)对大鼠肺泡巨噬细胞吞噬功能的影响

Impact of dust storm PM_(2.5)and PM_(10) on the phagocytic function of alveolar macrophages of rat

目的 探讨了沙尘暴PM2 5、PM10 对大鼠肺泡巨噬细胞吞噬功能的影响。方法 沙尘暴颗粒物采集自北京市城区 ,细胞毒性测定用MTT法 ,巨噬细胞吞噬功能采用流式细胞法 ,以荧光标记的胶珠 (Latexbeads ,D =2 4 μm)来测定。 结果 MTT结果显示 ,随着染毒浓度的增加 ,巨噬细胞存活率逐渐减低 ,高剂量组约为对照的 85 %左右。流式细胞仪分析表明 ,各颗粒物染毒组细胞的平均荧光强度逐渐减弱 ,提示巨噬细胞摄入沙尘暴颗粒物后 ,其吞噬荧光胶珠的能力明显减低 ;另外各染毒组的平均侧向光散射强度 ,即摄入的总颗粒数 (沙尘暴颗粒 +荧光胶珠 )低于对照 ,提示巨噬细胞的吞噬功能受到了损伤。沙尘暴PM2 5的细胞毒性比相同浓度的PM10 大 ,对吞噬功能的损伤也更明显。结论 沙尘暴PM2 5、PM10 均能损伤大鼠肺泡巨噬细胞的吞噬功能 ,削弱其非特异性防御能力。

Objective to investigated effects of dust storm PM 2 5 and PM 10 on the phagocytosis of rat alveolar macrophages. Methods\ PM 2 5 and PM 10 were collected during dust storm period in the urban area of Beijing. Cytotoxicity of PM 2 5 and PM 10 was measured by MTT method. Flow cytometry analysis was applied to characterize effects of PM 2 5 and PM 10 on the phagocytosis of alveolar macrophages. Results\ PM 2 5 and PM 10 were toxic to rat alveolar macrophages at the concentrations above 20μg/ml. PM 2 5 and PM 10 impaired phagocytic function of alveolar macrophages in a dose dependent manner. PM 2 5 was more potent than PM 10 Conclusion\ Dust storm PM 2 5 and PM 10 could impair the phagocytosis of rat alveolar macrophages, that may in turn impair the airway nonspecific defenses function.