碘氟联用对大鼠脑细胞膜磷脂和脂肪酸组成的影响

Influence of combined iodine and fluoride on phospholipid and fatty acid composition in brain cells of rats

目的 研究不同浓度碘和高浓度氟联合作用对大鼠脑细胞膜磷脂和脂肪酸组成的影响。方法 给大鼠饮用高浓度的氟化钠 (15 0mg L) ,同时分别加入低、中、高浓度碘化钾(0 0 0 3、0 0 3和 3mg L)的溶液共 5个月 ,然后用液相色谱法分离和测定大鼠脑细胞磷脂和脂肪酸组成。结果 实验各组中各种磷脂含量无显著变化。高浓度氟和高浓度碘加氟 (15 0mg L + 3mg L)可使大鼠脑细胞膜脂肪酸构成比发生了明显的变化 ,饱和脂肪酸中的月桂酸(C12 :0 )显著增加 (P <0 0 5 ) ,不饱和脂肪酸中的亚油酸 (C18:2 )显著减少 ;中浓度碘对氟的这种作用有明显的拮抗作用 ,而高浓度时则反而有协同毒性作用。结论 氟中毒所致大鼠脑细胞膜脂肪酸组成的变化可能与氟致脂质过氧化作用有关 ,而 0 0 3mg

Objective Investigating the influence of combined iodine and fluoride on phospholipid and fatty acid composition in brain cells of rats. Methods Five groups of rats were provided with deionized drinking water containing 0 and 150 mg/L NaF,and containing both 150mg/L NaF and 0 003,0 03 or 3 mg/L KI respectively for 5 months. Then phospholipid and fatty acid composition were determined using liquid chromatography. Results\ The phospholipid composition had no obvious change. The high concentration fluoride (150mg/L)and high concentration Iodine (3mg/L)with high concentration fluoride could cause significant changes of the fatty acid composition in brain cells of rats, the proportion of unsaturated fatty acid (C18:2) was significantly decreased and the saturated fatty acid(C12:0)increased obviously. The antagonistic action of 0 03 mg/L KI drinking water on this kind of influence induced by 150 mg/L NaF was the most evident, whereas that of 3mg/L KI was action of synergetic toxicity. Conclusion\ Fluorosis had obvious influence on phospholipid and fatty acid composition in brain cells of rats, and its mechanism might be associated with action of lipid peroxidation, and 0 03 mg/L KI is the optimal concentration for the antagonistic action with this influence from fluorosis.