牛磺酸对高同型半胱氨酸鸡胚心脏畸形发生的作用

Effects of taurine on the heart defects in chick embryos which induced by homocysteine

目的 探讨牛磺酸对高同型半胱氨酸 (HCY)鸡胚心脏发育及血管生成的影响。方法 种蛋经 1‰新洁尔灭清洗 ,分批入孵化箱 ,在 37 5℃、相对湿度 6 0 %的环境条件下培养。3天后用照蛋器检出活胚 ,随机分 3组 ,经卵黄囊给药 :(1)阴性对照组注射PBS ,(2 )HCY组注射HCY ,(3)牛磺酸组注射HCY +牛磺酸 ,继续培养 2d后组织病理检查。结果 HCY组器官形成期的心脏出现明显致畸 ,卵黄囊血管生成受到抑制 (P <0 0 5 ) ,心脏结构紊乱。牛磺酸的干预作用明显 ,有效的预防了HCY在器官形成期对心脏的致畸作用。结论 牛磺酸可防治HCY所引起的心脏发育障碍。

Objective To explore the effects of taurine on the heart defects in chick embryos induced by homocysteine (HCY). Methods Through the teratogenicity test of Chick Embryos, the taurine and HCY were given to yolk sac of 3 day's chick embryos. Histopathological examination after another 2 days culture. Results The occurrence of heart defects on HCY group were significantly increased. The growth of vitelline vessels was decreased. The prevention of tautine on the heart defects in chick embryos induced by HCY was very significantly effective. Conclusion Taurine can prevent the developmental disturbance of hearts induced by HCY in this experiment.