乙醇醛对小鼠脾淋巴细胞DNA损伤作用的研究

Study of glycolaldehyde on DNA damage in mouse spleen lymphocytes

目的 观察乙醇醛对DNA的损伤作用。方法 采用单细胞凝胶电泳 (SCGE)技术检测不同浓度乙醇醛对小鼠脾淋巴细胞DNA的损伤程度。结果 当乙醇醛浓度为 0 0 1mmol L时即能引起脾淋巴细胞DNA链断裂损伤 ,DNA损伤率为 8% ,DNA平均迁移距离为 ( 14 32± 2 84 ) μm。随着乙醇醛浓度的升高DNA损伤率和DNA平均迁移距离均以浓度依赖方式增加。

Objective Study the effect of glycolaldehyde on DNA of mouse spleen lymphocytes. Methods The DNA damage induced by different concentrations of glycolaldehyde was detected using single cell gel electrophoresis assay (SCGE). Results Glycolaldehyde at 0 01mmol/L could induce lymphocyte DNA strand breakage and DNA damage rate was 8% and, the average migration distance of DNA was (14 32±2 84)μm. DNA damage rate and the average migration distance of DNA were increased in a concentration dependent manner. Conclusion glycolaldehyde could induce DNA damage in different concentration in this experiment.