某健齿露防龋功能性评价的实验研究

Protection of tooth protecting spray against dental carries

目的 评价某健齿露对龋齿的预防作用。方法 离体试验 :将一定浓度的样品加入变形链球菌培养管中 ,培养一定时间 ,经革兰氏阳性染色和分光光度计测量观察变形链球菌的生长情况 ,测定其百分粘附率 ,并用酸度计测定细菌产酸后pH值。动物试验 :采用变形链球菌建立大鼠龋损模型 ,设立空白对照组 ,低、中、高三个剂量组 ,每日给样 ,连续 4 0天后处理动物 ,观察各组大鼠磨牙患龋情况 ,参照keyes经典龋齿计分标准进行龋齿计分。结果 变形链球菌生长正常 ,但易形成长链 ,粘附率降低。pH值升高 ,大于 6。动物实验表明 :实验组与对照组龋损程度、龋齿计分之间差异有显著性 (P <0 0 1)。结论 该样品能减少龋损的形成 ,有效降低大鼠龋病的发生、发展 ,有一定的防龋功能。其防龋机理在于该样品可促进变形链球菌凝集 ,抑制变形链球菌在玻璃上的粘附 ,降低变形链球菌产酸。

Objective To investigate the growth, adherence and soul production of streptococcus mutans incubated with olitin tooth protecting spray and the effect of specific sample against dental caries. Methods Streptococcus mutans were cultured with the sample of Olitin tooth protecting spray. After culturing, S. mutans were observed by Gram stain and by spectrophotometer, and the percentages of the adherent S. mutans were calculated. 40 Wistar rats were divided into 4groups: group A was control group; group B,C,D was low, middle, high test group respectively. All test groups received samples. The sum of the caries scores was assessed by the Keyes' procedure. Results The sample did not inhibit the growth of S. mutans , but the long chain was occurred. The adherence of S. mutans cells on the glass was decrease. the pH value was increase to above 6 0. There was a significant lower mean of caries score of test group than control group. Conclusion The sample can prevent the development of dental caries in this experiment. The mechanism may relate with the decrease of adherence of S. mutans .