离体条件下暴马丁香切开种子的萌发

In vitro germination of cut seeds of Syringa reticulata var. mandshurica

分别以三种催芽处理 1 0、2 0、40d的暴马丁香种子进行离体培养试验 ,对种子做三种切割处理 ,中间切、两端切和完整种子。结果表明 :采用切开种子的方法进行离体培养可提高种子发芽率 ,中间切种子的萌发最好 ,且在培养 1 0d后发芽率即达到最高值 ;经过 40d催芽处理的种子优于未经催芽和只进行 2 0d催芽处理的种子 ;切开种子以MS培养基附加 5mg/LBA或 5mg/LBA + 0 .

Seeds of Syringa reticulata var. mandshurica stratified for 10, 20 and 40 days were in vitro cultured. Before incubation, three different treatments of seeds were conducted: intact, middle-cut, two ends-cut. The results showed that germination percentage of cut seed was enhanced, middle-cut was the best and achieved maximum germination after 10 days culture; germination of seeds treated 40 days was better than that of the seeds with other two treatments. In vitro germination of seeds with 40 days stratification was best on MS medium with 5 mg/L BA or BA 5 mg/L BA+0.1 mg/L IBA.