我国鼠疫耶尔森菌最大质粒的研究

The Study of the Largest Plasmids of Yersinia pestis in China

目的 研究我国鼠疫菌最大质粒的特性及地理分布特征。方法 采用Kado和Liu法提取我国各鼠疫自然疫源地 2 0 2 6株鼠疫菌的质粒 ,经琼脂糖凝胶电泳进行检测分析。结果 我国鼠疫菌具有分类属性的最大质粒有 3种 ,其相对分子质量分别为 5 2× 10 6、65× 10 6和 90× 10 6,有明显的地理分布特征。 5 2× 10 6质粒分布在祁连山南、北麓和青海湖环湖地区 ,90× 10 6质粒仅分布在藏北高原的那曲地区和青海青南高原的玉树、曲麻莱、格尔木的部分地区 ,其余各鼠疫疫源地的鼠疫菌其最大质粒均为 65× 10 6。结论 我国鼠疫菌最大质粒的特点及地理分布对研究鼠疫自然疫源地空间结构和鼠疫菌的遗传学特性具有重要意义。

Objective To study the characteristics and the geographic distribution of Yersinia pesits strains with the largest plasmids in China. Methods According to the methods of Kado and Liu, the plasmids were extracted from 2 026 Y.pestis strains from the plague natural foci and analyzed by Agar Gel Electrophoresis. Results The strains with the largest plasmid are carried three kinds, and the relative molecular weight are 52×106, 65×106 and 90×106. Strains with 52×106 plasmid are distributed in the southern and northern foothills of the Qilian Mountains and areas around the Qinghai Lake. Strains with 52×106 plasmid are only found in the Naqu areas of the northern Tibetan Plateau and the part areas of Yushu, Qumalai County and Geermu City. Strains with 65×106 plasmid are distributed in the other plague natural foci in China. Conclusion The studies of characteristics and the geographical distribution of strains with the largest plasmid are of great significance to analyze the space construction of the plague natural foci and the genetics characteristics of Y.pestis in China.