不同地理株埃及伊蚊经口接种登革2型病毒的感染性研究

Study Among Different Strains of Aedes aegypti in Susceptibility for Oral Infection with DEN-2 Virus

目的 研究海南、广东、台湾和印尼Baro等 4个地理株埃及伊蚊对登革 2型病毒的感染情况。方法 分别用鼠血和猪血配制不同病毒滴度的病毒混悬液经口接种埃及伊蚊 ,间接免疫荧光检测蚊脑液病毒抗原。结果 用鼠血病毒混悬液时 ,印尼Baro株对登革 2型病毒的感染率与海南株、台湾株的感染率差异有显著性 (海南株 :P =0 .0 0 79<0 .0 1;台湾株 :P =0 .0 116<0 .0 5 ) ,与广东株的感染率差异无显著性 (P >0 .0 5 ) ,随着病毒滴度的下降 ,各株埃及伊蚊对病毒的感染率呈明显下降趋势 ;鼠血和猪血各株饱血雌蚊经口接种登革 2型病毒的感染率差异无统计学意义 (χ2 =1.63 2 0 ,P =0 .2 0 14 >0 .0 5 )。结论 不同地理株埃及伊蚊对登革 2型病毒易感性存在差异。

Objective To study the susceptibility of Aedes aegypti to DEN-2 virus among 4 geographic strains from China and Baro of Indonesia. Methods Aedes aegypti of different geographic population were infected with dengue 2 virus through oral and indirect fluoroimmunoassay was used to detect antigen the virus in brain of Aedes aegypti. Results (1) Using mice- blood-sugar virus suspension, the infection rate of Baro strain to dengue 2 virus shows significant variation with Hainan strain's ( P= 0.007 9< 0.01) and Taiwan strain's ( P= 0.011 6< 0.05). But no significant variation with Guangdong strain's ( P> 0.05). (2) With the reduction of virus titre (TCID 50), the infection rate of every geographic strain to dengue 2 virus shows extraordinarily marked downtrend. (3) No significant difference has shown in susceptibility of every strain of full-blood by mice and pig- blood-sugar virus suspension. Conclusion The infecting effects of different geographic strains of Aedes aegypti to dengue 2 virus show significant difference.