白阿魏菇菌丝体多糖(CPNMP)的药理作用

Effect of the Mycelium Polysaccharides from Pleurotus Nebrodensis on Parmacological Function

用沸水提取白阿魏菇菌丝体多糖 ,经除蛋白 ,醇析得粗多糖 (CPNMP) ,含多糖 5 9.2 % 进行免疫、抗炎和抗肿瘤实验 ,结果 :CPNMP能增加中性红吞噬指数 (P <0 .0 5 ) ,促进DTH的发生 (P <0 .0 1) ,促进红细胞SOD(P <0 .0 1) ,血清SOD(P <0 .0 1)、CAT(P <0 .0 1)和溶菌酶活力 (P <0 .0 1) ,降低MDA(P <0 .0 5 ) ,而且对胆固醇有促进作用 (P<0 .0 5 ) ;CPNMP能明显抑制二甲苯所致小鼠耳肿胀 ,抑制小鼠毛细血管通透性 ;CPNMP还能明显地抑制S180 的瘤重 ,抑瘤率为 39.1% ,提高血清SOD(P <0 .0 5 )、CAT(P <0 .0 1)溶菌酶 (P <0 .0 1)和LDH -L(P <0 .0 5 )的活力 ;增加免疫器官的重量 (P <0 .0 1) ,降低MDA(P <0 .0 5 )和ALP活力 (P <0 .0 5 ) .因此CPNMP有提高免疫力 ,抗炎。

Pleurotus nebrodensis mycelium polysaccharides (CPNMP) were extracted with boiling water method from pleurotus nebrodensis mycelium. The protein was removed from CPNMP for three times with Sevag method. To make experiments delayed-type hypersensitivity (DTH) induced by the 2-dinitr-chloride benzene (DNCB) and to test the absorption of neutral red, Serum haemolysin,SOD,CAT,MDA,lysozyme of serum and RBC SOD. And the effects of CPNMP on tumor weight and immune function were observed in S180-bearing mice. The results showed that CPNMP enhanced cellular immunity of mice significantly.,And the function of phagocytic and RBC. Increased the activation of SOD, CAT, lysozyme of serum and Cholesterol,And Inhibited humoral immunity. This could relate with its anti-inflammatory. CPNMP inhibited obviously swelling ears of mice induced by xylene and decreased capillary permeability of mice induced by acetic acid. CPNMP could markedly decrease tumor weight and increase weight of immune organs. CPNMP also increased SOD?CAT and LDH-L activity in serum in S180-bearing mice. Decreased MDA and ALP in serum in mice.It shows that CPNMP can be anti-inflammatory and anti-tumor,and enhance immune function.